Recombinant expression and characterization of a reducing-end xylose-releasing exo-oligoxylanase from Bifidobacterium adolescentis

Lagaert, Stijn; Van Campenhout, Steven; Pollet, Annick; Bourgois, Tine; Delcour, Jan; Courtin, Christophe; Volckaert, Guido

doi:10.1128/AEM.00722-07

Recombinant expression and characterization of a reducing-end xylose-releasing exo-oligoxylanase from Bifidobacterium adolescentis

Author:

Lagaert, Stijn

Van Campenhout, Steven ; Pollet, Annick ; Bourgois, Tine ; Delcour, Jan ; Courtin, Christophe ; Volckaert, Guido

Keywords:

xylooligosaccharides, oligosaccharides, Bifidobacterium adolescentis, Science & Technology, Life Sciences & Biomedicine, Biotechnology & Applied Microbiology, Microbiology, BACILLUS-HALODURANS C-125, XYLOOLIGOSACCHARIDES, OLIGOSACCHARIDES, RATS, Amino Acid Sequence, Bacterial Proteins, Bifidobacterium, Electrophoresis, Polyacrylamide Gel, Glycoside Hydrolases, Hydrogen-Ion Concentration, Hydrolysis, Molecular Sequence Data, Phylogeny, Recombinant Proteins, Sequence Homology, Amino Acid, Temperature, Xylose, 3107 Microbiology, 3207 Medical microbiology

Abstract:

The family 8 glycoside hydrolase (RexA) from Bifidobacterium adolescentis was expressed in Escherichia coli. The recombinant enzyme was characterized as a reducing-end xylose-releasing exo-oligoxylanase. Apart from giving insights into this new class of enzymes, knowledge of the RexA enzyme helps to postulate a mechanism for the B. adolescentis breakdown of prebiotic xylooligosaccharides.