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npj Breast Cancer

Publication date: 2024-04-24
Volume: 10
Publisher: Nature Portfolio

Author:

Geukens, Tatjana
De Schepper, Maxim ; van den Bogaert, Wouter ; Van Baelen, Karen ; Maetens, Marion ; Pabba, Anirudh ; Mahdami, Amena ; Leduc, Sophia ; Isnaldi, Edoardo ; Nguyen, Ha-Linh ; Bachir, Imane ; Hajipirloo, Maysam ; Zels, Gitte ; Van Cauwenberge, Josephine ; Borremans, Kristien ; Vandecaveye, Vincent ; Weynand, Birgit ; Vermeulen, Peter ; Leucci, Eleonora ; Baietti, Maria Francesca ; Sflomos, George ; Battista, Laura ; Brisken, Cathrin ; Derksen, Patrick WB ; Koorman, Thijs ; Visser, Daan ; Scheele, Colinda LGJ ; Thommen, Daniela S ; Hatse, Sigrid ; Fendt, Sarah-Maria ; Vanderheyden, Evy ; Van Brussel, Thomas ; Schepers, Rogier ; Boeckx, Bram ; Lambrechts, Diether ; Marano, Giuseppe ; Biganzoli, Elia ; Smeets, Ann ; Nevelsteen, Ines ; Punie, Kevin ; Neven, Patrick ; Wildiers, Hans ; Richard, Francois ; Floris, Giuseppe ; Desmedt, Christine

Keywords:

Science & Technology, Life Sciences & Biomedicine, Oncology, PROGESTERONE-RECEPTOR, GENE-EXPRESSION, PRIMARY TUMORS, COLD ISCHEMIA, BREAST, HETEROGENEITY, PROCUREMENT, ESTROGEN, MODEL, TIME, G0B4122N#56765319, G011724N#57877936, C14/22/137#57006245, 1S76520N|1S76522N#55416700, 1297322N#56489472, 1802222N#56524804, G0B6120N#55523387, G093821N#56129096, C14/18/092#54689607, C14/22/125#57006238, G059821N#56124601, C14/21/114#56287027, 3202 Clinical sciences, 3211 Oncology and carcinogenesis, 4202 Epidemiology

Abstract:

Research on metastatic cancer has been hampered by limited sample availability. Here we present the breast cancer post-mortem tissue donation program UPTIDER and show how it enabled sampling of a median of 31 (range: 5-90) metastases and 5-8 liquids per patient from its first 20 patients. In a dedicated experiment, we show the mild impact of increasing time after death on RNA quality, transcriptional profiles and immunohistochemical staining in tumor tissue samples. We show that this impact can be counteracted by organ cooling. We successfully generated ex vivo models from tissue and liquid biopsies from distinct histological subtypes of breast cancer. We anticipate these and future findings of UPTIDER to elucidate mechanisms of disease progression and treatment resistance and to provide tools for the exploration of precision medicine strategies in the metastatic setting.