Author:

Keywords:

Science & Technology, Life Sciences & Biomedicine, Biochemical Research Methods, Oncology, Immunology, Medicine, Research & Experimental, Biochemistry & Molecular Biology, Research & Experimental Medicine, HUMAN-BLOOD, MICE, ENGRAFTMENT, CELLS, Humanization, Immunotherapy, In vivo, PDX, Tumor models, Xenografts, Animals, Mice, Humans, Xenograft Model Antitumor Assays, Neoplasms, Disease Models, Animal, 0601 Biochemistry and Cell Biology, Developmental Biology, 3101 Biochemistry and cell biology

Abstract:

Patient-derived xenograft (PDX) models are the golden standard for preclinical oncology as they can recapitulate the genotypic and phenotypic complexity of human tumors, thus enabling the development of effective therapeutic strategies. PDX models are typically established in im- munocompromised animals that allow efficient growth of the xenografted tumor. Given the re- cent success of immune therapies in different tumors however, the establishment of humanized PDX models is critical to evaluate immune oncology drugs and/or combinations thereof. Here, we describe the detailed methods to obtain humanized PDX models for anti-cancer therapy testing.