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Cell Stem Cell

Publication date: 2022-12-01
Volume: 29 Pages: 1685 -
Publisher: Cell Press

Author:

Pantazis, Caroline B
Yang, Andrian ; Lara, Erika ; McDonough, Justin A ; Blauwendraat, Cornelis ; Peng, Lirong ; Oguro, Hideyuki ; Kanaujiya, Jitendra ; Zou, Jizhong ; Sebesta, David ; Pratt, Gretchen ; Cross, Erin ; Blockwick, Jeffrey ; Buxton, Philip ; Kinner-Bibeau, Lauren ; Medura, Constance ; Tompkins, Christopher ; Hughes, Stephen ; Santiana, Marianita ; Faghri, Faraz ; Nalls, Mike A ; Vitale, Daniel ; Ballard, Shannon ; Qi, Yue A ; Ramos, Daniel M ; Anderson, Kailyn M ; Stadler, Julia ; Narayan, Priyanka ; Papademetriou, Jason ; Reilly, Luke ; Nelson, Matthew P ; Aggarwal, Sanya ; Rosen, Leah U ; Kirwan, Peter ; Pisupati, Venkat ; Coon, Steven L ; Scholz, Sonja W ; Priebe, Theresa ; Ottl, Miriam ; Dong, Jian ; Meijer, Marieke ; Janssen, Lara JM ; Lourenco, Vanessa S ; van der Kant, Rik ; Crusius, Dennis ; Paquet, Dominik ; Raulin, Ana-Caroline ; Bu, Guojun ; Held, Aaron ; Wainger, Brian J ; Gabriele, Rebecca MC ; Casey, Jackie M ; Wray, Selina ; Abu-Bonsrah, Dad ; Parish, Clare L ; Beccari, Melinda S ; Cleveland, Don W ; Li, Emmy ; Rose, Indigo VL ; Kampmann, Martin ; Aristoy, Carles Calatayud ; Verstreken, Patrik ; Heinrich, Laurin ; Chen, Max Y ; Schule, Birgitt ; Dou, Dan ; Holzbaur, Erika LF ; Zanellati, Maria Clara ; Basundra, Richa ; Deshmukh, Mohanish ; Cohen, Sarah ; Khanna, Richa ; Raman, Malavika ; Nevin, Zachary S ; Matia, Madeline ; Van Lent, Jonas ; Timmerman, Vincent ; Conklin, Bruce R ; Chase, Katherine Johnson ; Zhang, Ke ; Funes, Salome ; Bosco, Daryl A ; Erlebach, Lena ; Welzer, Marc ; Kronenberg-Versteeg, Deborah ; Lyu, Guochang ; Arenas, Ernest ; Coccia, Elena ; Sarrafha, Lily ; Ahfeldt, Tim ; Marioni, John C ; Skarnes, William C ; Cookson, Mark R ; Ward, Michael E ; Merkle, Florian T

Keywords:

Science & Technology, Life Sciences & Biomedicine, Cell & Tissue Engineering, Cell Biology, DIRECTIONAL GENOMIC HYBRIDIZATION, COPY NUMBER, DOPAMINE NEURONS, RISK LOCI, A-BETA, DIFFERENTIATION, GENERATION, DISEASE, GENE, HETEROGENEITY, CRISPR, differentiation, iPSC, karyotype, p53, pluripotent, reference, single-cell, stem cell, whole-genome, Humans, Induced Pluripotent Stem Cells, Cell Differentiation, Gene Editing, Biological Assay, G0B8119N#54971224, 06 Biological Sciences, 11 Medical and Health Sciences, Developmental Biology, 31 Biological sciences, 32 Biomedical and clinical sciences

Abstract:

Human induced pluripotent stem cell (iPSC) lines are a powerful tool for studying development and disease, but the considerable phenotypic variation between lines makes it challenging to replicate key findings and integrate data across research groups. To address this issue, we sub-cloned candidate human iPSC lines and deeply characterized their genetic properties using whole genome sequencing, their genomic stability upon CRISPR-Cas9-based gene editing, and their phenotypic properties including differentiation to commonly used cell types. These studies identified KOLF2.1J as an all-around well-performing iPSC line. We then shared KOLF2.1J with groups around the world who tested its performance in head-to-head comparisons with their own preferred iPSC lines across a diverse range of differentiation protocols and functional assays. On the strength of these findings, we have made KOLF2.1J and its gene-edited derivative clones readily accessible to promote the standardization required for large-scale collaborative science in the stem cell field.