Author:

Keywords:

Science & Technology, Physical Sciences, Chemistry, Analytical, Chemistry, Dual immunoplatform, BAFF, APRIL, MoS2/MWCNTs, Amperometry, Serum, SLE, CRC, IL-13 RECEPTOR ALPHA-2, CARBON NANOTUBES, FAMILY, Antibodies, Biosensing Techniques, Cell Proliferation, Cytokines, Electrochemical Techniques, Humans, Hydrogen Peroxide, Immunoassay, Molybdenum, Nanostructures, 0301 Analytical Chemistry, 0399 Other Chemical Sciences, Analytical Chemistry, 3401 Analytical chemistry

Abstract:

A dual immunosensor is reported for the simultaneous determination of two important immunity-related cytokines: BAFF (B cell activation factor) and APRIL (a proliferation-induced signal). Sandwich-type immunoassays with specific antibodies (cAbs) and a strategy for signal amplification based on labelling the detection antibodies (dAbs) with binary MoS2/MWCNTs nanostructures and using horseradish peroxidase (HRP) were implemented. Amperometric detection was carried out at screen-printed dual carbon electrodes (SPdCEs) through the hydroquinone HQ/H2O2 system. The developed dual immunosensor provided limit of detection (LOD) of 0.08 and 0.06 ng mL-1 for BAFF and APRIL, respectively, and proved to be useful for the determination of both cytokines in cancer cell lysates and serum samples from patients diagnosed with autoimmune diseases and cancer. The obtained results agreed with those found using ELISA methodologies.