Author:

Keywords:

Science & Technology, Life Sciences & Biomedicine, Obstetrics & Gynecology, Reproductive Biology, dysphasia of the fibrous sheath, hypo-osmotic swelling test, immotile cilia syndrome, intracytoplasmic sperm injection, Kartagener's syndrome, INTRACYTOPLASMIC SPERM INJECTION, HYPOOSMOTIC SWELLING TEST, KARTAGENERS-SYNDROME, SUBZONAL INSEMINATION, FERTILIZATION RATES, MEN, SELECTION, FEATURES, BIRTH, Adult, Ciliary Motility Disorders, Ejaculation, Female, Humans, Male, Oligospermia, Osmosis, Pregnancy, Severity of Illness Index, Sperm Count, Sperm Injections, Intracytoplasmic, Sperm Motility, Spermatozoa, 1114 Paediatrics and Reproductive Medicine, Obstetrics & Reproductive Medicine, 3215 Reproductive medicine

Abstract:

This study presents a case of intracytoplasmic sperm injection (ICSI) with ejaculated immotile spermatozoa from a patient with immotile cilia syndrome. Semen analysis of the patient suffering from immotile cilia syndrome revealed an extreme oligoasthenoteratozoospermia (OAT: count <1.4 x 10(6)/ml, 0% motility and 3% normal morphology). Electron microscopy of sperm flagella showed the absence of inner and outer dynein arms. During the ICSI cycle, the hypo-osmotic swelling test (HOS) was used for the identification of viable spermatozoa in the pool of immotile spermatozoa for ICSI. A normal fertilization rate was found in eight out of the 12 oocytes. A first fresh double embryo transfer resulted in a late miscarriage at 21 weeks. A second healthy singleton pregnancy occurred after transfer of two frozen-thawed embryos from the same ICSI procedure. Although only one successful ICSI case of the immotile cilia syndrome combined with HOS is described here, HOS might be a simple but valuable tool to obtain normal fertilization and pregnancy for patients suffering from immotile spermatozoa.