Author:

Keywords:

Science & Technology, Life Sciences & Biomedicine, Endocrinology & Metabolism, Type 2 diabetes, Roux-en-Y gastric bypass, Mandibular condyle, Bone composition, Cancellous bone, Raman spectroscopy, NONENZYMATIC GLYCATION, RAMAN-SPECTROSCOPY, MECHANICAL-PROPERTIES, MANDIBULAR CONDYLE, DIABETES-MELLITUS, MINERAL DENSITY, ALVEOLAR BONE, CONSISTENCY, STRENGTH, TYPE-1, Animals, Bone and Bones, Diabetes Mellitus, Type 2, Gastric Bypass, Male, Mice, Mice, Inbred C57BL, Obesity, Random Allocation, 0601 Biochemistry and Cell Biology, 0903 Biomedical Engineering, 1103 Clinical Sciences, 3202 Clinical sciences, 4003 Biomedical engineering

Abstract:

Obesity is the main cause of type 2 diabetes mellitus (T2DM). Roux-en-Y gastric bypass (RYGB) surgery is an effective treatment for this obesity-related health problem. However, the adverse effects of T2DM on bone tissue persist or even aggravate after this surgical procedure. As studies on the mandibular condyle bone are scarce, the aim of the present study was to assess its compositional characteristics in T2DM and RYGB conditions. Thirty-two male C57BL/6 mice at 8 weeks of age were randomly assigned to receive either a high-fat or low-fat diet. After 14 weeks of high-fat diet intake, seven obese mice were subjected to RYGB surgery. All animals were euthanized at the age of 30 weeks. Mandibular bones were removed and the trabecular condyle region was assessed using Raman spectroscopy. A decreased mineralization was observed for both T2DM and RYGB condyle bones when compared to controls, with elevated carbonate substitutions for the RYGB group. No compositional differences in crystallinity and presence of advanced glycation end products were found between the groups, with the exception of an increased presence of N-carboxymethyl-lysine in RYGB bone compared to their T2DM counterpart. Site-specific measurements revealed a non-uniform bone composition, with increasing mineralization and carbonate substitutions towards the centre of the mandibular condyle. T2DM and RYGB surgery affect the mandibular condyle bone quality, as investigated at compositional level. Assessment of bone structural properties and remodelling should be carried out to further explore the effects of T2DM and RYGB surgery on this skeleton area.