Author:

Keywords:

Animals, Cadaverine, Chromatography, High Pressure Liquid, Dansyl Compounds, Diamines, Fishes, Histamine, Indicators and Reagents, Meat, Putrescine, Spermidine, Spermine, Science & Technology, Life Sciences & Biomedicine, Food Science & Technology, Food Science

Abstract:

A simple and rapid method is described for the extraction, derivatization and subsequent determination by means of high pressure liquid chromatography of putrescine, cadaverine, histamine, spermidine and spermine as their 5-dimethylaminonaphthalene-1-sulphonyl derivatives. The amines are extracted from the fish material by an aqueous trichloroacetic acid solution and derivatized by means of 5-dimethylaminonaphthalene-1-sulphonylchloride (dansyl chloride). The reaction mixture is then injected on a RP-8 column using gradient elution to separate the amine derivatives. Use of the method results in a considerable saving with respect to both time and costs.