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Abstract:

Objectives: Previous results suggest that the indirect effect of CDV on an untreated secondary HPV positive tumor in mice could be related to the stimulation of B-cells, γδ-T-cells and NK-cells. Therefore, the activation of these immune cells was investigated. Methods: Mice were injected with SiHa cells (tumor 1, T1) and T2 was injected after three weeks of intratumoral treatment of T1 with CDV, adjuvant (aluminum-0.5% + MPLA-0.05%), PBS or a combination. Flow cytometry analysis of immune cells in spleen and tumors was performed. Levels of IgG and IgM in serum were evaluated by ELISA. IL17 and IFN-γ, both human and mice, are currently under investigation by means of ELISA. Results: An increased amount of γδ-T cells, NK-cells, and B-cells in spleen and T1 of mice treated with CDV compared to the groups treated with PBS was observed. In addition, a higher percentage of B-cells, γδ-T-cells and NK-cells was observed in T2 of mice treated with CDV plus adjuvant than in T2 of mice that only received CDV. Levels of IgG were increased in the serum of mice that received adjuvant. Levels of IgM were comparable between groups except for a low level in control mice. IgM concentration increased over time. Conclusion: Since levels of IgM increased over time, IgM production could be a reaction to the tumor. On the other hand, IgG could be produced in response to the combination of adjuvant injection and injection of the secondary tumor. This will be investigated further.