Author:

Abstract:

The RasV12/scrib-/- tumour model has been widely used in Drosophila to study Ras-dependant oncogenesis and epithelial-mesenchymal transition (EMT). Recently, using a combination of ATAC-seq, FAIRE-seq and RNA-seq, we identified regulatory regions and two key transcription factors (AP-1 and Stat92E) involved in the development of these tumours. Using data from the whole tumour, it is impossible to determine whether these factors are part of the same gene regulatory networks (GRN) in a cell, or if they are controlling separate GRNs in different cells. In an attempt to answer this question, we used the C1 single cell auto-prep system (Fluidigm) and performed single-cell RNA-seq (SMART-seq2) and single-cell ATAC-seq on dissociated in vivo tumours. We envision that this approach will help answer this question and help enable us to start understand GRNs on the single cell level.