Author:

Keywords:

antarctic lakes, microbial mats, gen. nov., bacteria, hybridization, soil, dna, Science & Technology, Life Sciences & Biomedicine, Microbiology, ANTARCTIC LAKES, MICROBIAL MATS, GEN. NOV., BACTERIA, HYBRIDIZATION, SOIL, DNA, Antibiosis, Araceae, Base Composition, Catalase, Culture Media, DNA, Bacterial, Fatty Acids, Gammaproteobacteria, Hypocreales, Molecular Sequence Data, Oxidoreductases, Phylogeny, Plant Diseases, Plant Roots, RNA, Bacterial, RNA, Ribosomal, 16S, Sequence Homology, Nucleic Acid, Soil, Soil Microbiology, Species Specificity, 0603 Evolutionary Biology, 0605 Microbiology, 1108 Medical Microbiology, 3104 Evolutionary biology, 3107 Microbiology

Abstract:

Three Gram-negative, yellow-pigmented strains were isolated from the rhizospheres of Spathiphyllum plants grown in a compost-amended potting mix. The strains showed biological control activity towards the root-rot plant pathogen Cylindrocladium spathiphylli, and were characterized to determine their taxonomic position. Cells of the strains were non-motile rods, and the strains were oxidase- and catalase-positive and unable to ferment most sugars tested. The three strains showed differences in growth temperature range, optimal growth temperature and some biochemical reactions. The majority of the fatty acids were branched, and large amounts of 15 : 0 iso and 17 : 1 iso v9c were present. The 16S rRNA gene sequence (1497 bp) of strain B39T showed the highest level of similarity (98?5 %) to that ofRhodanobacter fulvus IAM 15025T, followed byRhodanobacter lindaniclasticus LMG 18385T (96?0 %; strain no longer extant), Dyella koreensis CCUG 50883T (96?4 %), Dyella japonica DSM 16301T (96?3 %), Frateuria aurantia LMG 1558T (96?2 %) andFulvimonas soliLMG 19981T (95?9 %). Less than 90 % 16S rRNA gene sequence similarity was observed for other members of the Gammaproteobacteria. The mean DNA–DNA reassociation value for the three strains was 100 % and was 25 % when the strains were compared with DNA fromR. fulvus LMG 23003T . The strains had a mean DNA G+C content of 67?6 mol%. On the basis of their phylogenetic, genomic and phenotypic properties, the three strains represent a novel species within the genus Rhodanobacter, for which the name Rhodanobacter spathiphylli sp. nov. is proposed. The type strain is strain B39T (=LMG 23181T =DSM 17631T ).