Author:

Keywords:

Endothelin-1; Cardiac hypertrophy; Signalling; MAPK; GPCRs, Science & Technology, Life Sciences & Biomedicine, Cell Biology, Endothelin-1, Cardiac hypertrophy, Signalling, MAPK, GPCRs, LEFT-VENTRICULAR HYPERTROPHY, BRAIN NATRIURETIC PEPTIDE, GENE-EXPRESSION, CARDIOVASCULAR-DISEASE, REGULATED KINASES, HEART-FAILURE, BETA-ARRESTIN, CA2+ RELEASE, REGRESSION, AGONISTS, Animals, Animals, Newborn, Cardiomegaly, Gene Expression Regulation, HEK293 Cells, Humans, Male, Myocytes, Cardiac, Phenylephrine, Protein Kinase Inhibitors, RNA, Messenger, Rats, Wistar, Receptor, Endothelin A, Signal Transduction, Transcription, Genetic, Up-Regulation, 0601 Biochemistry and Cell Biology, 1116 Medical Physiology, Biochemistry & Molecular Biology, 3101 Biochemistry and cell biology

Abstract:

G-protein coupled receptor (GPCR) mediated activation of the MAPK signalling cascade is a key pathway in the induction of hypertrophic remodelling of the heart - a response to pathological cues including hypertension and myocardial infarction. While levels of pro-hypertrophic hormone agonists of GPCRs increase during periods of greater workload to enhance cardiac output, hypertrophy does not necessarily result. Here we investigated the relationship between the duration of exposure to the pro-hypertrophic GPCR agonist endothelin-1 (ET-1) and the induction of hypertrophic remodelling in neonatal rat ventricular myocytes (NRVM) and in the adult rat heart in vivo. Notably, a 15min pulse of ET-1 was sufficient to induce markers of hypertrophy that were present when measured at 24h in vivo and 48h in vitro. The persistence of ET-1 action was insensitive to ET type A receptor (ETA receptor) antagonism with BQ123. The extended effects of ET-1 were dependent upon sustained MAPK signalling and involved persistent transcription. Inhibitors of endocytosis however conferred sensitivity upon the hypertrophic response to BQ123, suggesting that endocytosis of ETA receptors following ligand binding preserves their active state by protection against antagonist. Contrastingly, α1 adrenergic-induced hypertrophic responses required the continued presence of agonist and were sensitive to antagonist. These studies shed new light on strategies to pharmacologically intervene in the action of different pro-hypertrophic mediators.