Author:

Keywords:

Science & Technology, Physical Sciences, Chemistry, Multidisciplinary, Chemistry, MOLECULAR RECOGNITION, COPPER(II) CATALYSTS, CLEAVAGE, RNA, COMPLEXES, IMIDAZOLE, PHOSPHODIESTERS, RIBONUCLEASES, MONOPHOSPHATE, DERIVATIVES, 03 Chemical Sciences, General Chemistry, 34 Chemical sciences, 40 Engineering

Abstract:

The rates of hydrolysis of two activated phosphate triesters, bis(p-nitrophenyl) methyl phosphate 3 and adenosine 5’-0, 0-bis(p-nitrophenyl) phosphate 4 have been studied using two copper-containing catalysts, Cu(Kemp-bpy) 1 and Cu(bpy) 2. The catalytic activities of these two species have been compared within the pH range of 6—9.5. The rates of hydrolysis of both substrates, 3 and 4, have been compared to the uncatalyzed reaction. This rate-enhancement is dependent on pH. In both cases, the monohydroxo H(OH-)+ species is more catalytically active than the diaqua species, H(H2O)2+. The effect of binding on overall rate acceleration was evaluated; the maximum contribution was found to be 44. © 1995, American Chemical Society. All rights reserved.