Author:

Keywords:

epstein-barr virus, lytic cycle, oxidative stress, lymphoblastoid b cell lines, manganese superoxide-dismutase, induced thymidine kinase, latently infected-cells, burkitts-lymphoma, free-radicals, lymphoproliferative disease, rheumatoid-arthritis, gene-expression, oxygen radicals, carrier state, Science & Technology, Life Sciences & Biomedicine, Cell Biology, Epstein-Barr virus, Lytic cycle, Oxidative stress, Lymphoblastoid B cell lines, FREE-RADICALS, INFECTIOUS-MONONUCLEOSIS, RHEUMATOID-ARTHRITIS, BURKITTS-LYMPHOMA, GENE-EXPRESSION, REPLICATION, ACTIVATION, GROWTH, BZLF1, IMMORTALIZATION, Antioxidants, Biomarkers, Cell Line, Tumor, Cell Transformation, Viral, Epstein-Barr Virus Infections, Herpesvirus 4, Human, Humans, Leukemia, B-Cell, Oxidants, Oxidative Stress, Oxidoreductases, 0601 Biochemistry and Cell Biology, Biochemistry & Molecular Biology, 3101 Biochemistry and cell biology

Abstract:

Here, we investigated the effect of induction of the Epstein-Barr virus (EBV) viral lytic cycle on the oxidant/antioxidant balance in three lymphoblastoid cell lines: B95-8, Raji, and LCL C1. The induction of the EBV lytic cycle was done by a non-stressing dose of 12-0-tetradecanoylphorbol-13-acetate (8 nM). Oxidative stress was assessed by measuring malondialdehyde as a parameter of lipid peroxidation, the levels of glutathione, and the activities of three antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase). After 48 h (peak of lytic cycle), a significant decrease in superoxide dismutase activity was observed in B95-8, Raji, and LCL C1 cells (P < 0.05). In addition, in B95-8 cells also a significant decrease of catalase activity was detected (P < 0.05). The glutathione peroxidase activity and the glutathione level were not significantly modified by the induction in any of the cell lines. We found a significant rise in malondialdehyde levels in B95-8, Raji, and LCL C1 cells after the induction of the lytic cycle compared to controls (P < 0.05). In conclusion, induction of EBV lytic cycle in lymphoblastoid cells causes increased oxidative stress in the host cells within 48 h, a process that could be involved in malignant transformations.