Author:

Keywords:

Acanthocytes, erythrocyte morphology, glomerular bleeding, Child, Erythrocytes, non-glomerular bleeding, microscopic examination, Fixatives, Formaldehyde, sediment, Glomerulonephritis, hematuria, Glomerulonephritis, IGA, Glomerulonephritis, Membranoproliferative, Hematuria, Humans, Microscopy, Phase-Contrast, Specimen Handling, Time Factors, Tissue Fixation, Science & Technology, Life Sciences & Biomedicine, Pediatrics, Urology & Nephrology, MICROSCOPIC EXAMINATION, SEDIMENT, HEMATURIA, 1114 Paediatrics and Reproductive Medicine, 3202 Clinical sciences, 3213 Paediatrics

Abstract:

Between 2 and 4 h after miction the morphology of urinary erythrocytes has changed, clouding the distinction between glomerular and non-glomerular bleeding in pediatric patients. Glomerular bleeding is characterized by microscopically visible alteration in urinary erythrocytes due to glomerular disease. Fixation by Cellfix, a formaldehyde-based fixative, allows the preservation of the morphology for at least 24 h and can be recommended for clinical practice. In our experience, thiomersal was not effective for preservation of the morphology of erythrocytes.