Author:

Keywords:

Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, Amino Acid Sequence, Base Sequence, Cell Line, Cloning, Molecular, DNA, Escherichia coli, Humans, Melanoma, Plasminogen Activators, Protein Conformation, General Science & Technology

Abstract:

Bacterial clones containing human tissue-type plasminogen activator (t-PA) cDNA sequences were identified in a cDNA library prepared using gel-fractionated mRNA from human melanoma cells. A plasmid containing the Escherichia coli trp promoter and the cDNA sequence coding f or the 527-amino acid mature t-PA protein was constructed for expression in E. coli. A polypeptide was produced having the fibrinolytic properties characteristic of authentic human t-PA. © 1983 Nature Publishing Group.