Author:

Keywords:

erythropoietin, glycophorin a, interleukin-3, metallothionein, zinc status, erythrocyte metallothionein, zinc-deficiency, human kidney, expression, isoform, induction, monocytes, proteins, binding, dna, Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, glycophorin A, ERYTHROCYTE METALLOTHIONEIN, ZINC-DEFICIENCY, HUMAN KIDNEY, EXPRESSION, ISOFORM, INDUCTION, MONOCYTES, PROTEINS, BINDING, DNA, Antigens, CD, Antigens, Differentiation, B-Lymphocyte, Cell Separation, Cells, Cultured, DNA, Complementary, Down-Regulation, Electrophoresis, Agar Gel, Erythrocytes, Erythropoietin, Fetal Blood, Glycophorins, Humans, Immunoblotting, Interleukin-3, Metallothionein, Poly A, Protein Isoforms, RNA, Messenger, Receptors, Transferrin, Transcription, Genetic, Zinc, 0304 Medicinal and Biomolecular Chemistry, 0601 Biochemistry and Cell Biology, 1101 Medical Biochemistry and Metabolomics, 3101 Biochemistry and cell biology, 3205 Medical biochemistry and metabolomics, 3404 Medicinal and biomolecular chemistry

Abstract:

The in vitro transcription patterns for 10 functional metallothionein (MT) isogenes have been investigated in red blood cell (RBC) precursors from human cord blood. Active transcription status of the isogenes, MT-0, MT-1A, MT-1B, MT-IE, MT-1G, MT-1X, and MT-2A, was detected in both ex vivo expanded RBC precursors (burst-forming unit-erythroid) and glycophorin A(+) and CD71(+) cells separated by magnetic cell sorting. Transcription patterns of these isogenes were analyzed at different times of incubation with the addition of Zn supplement. In neither the ex vivo expanded precursors nor glycophorin A(+) and CD71(+) cells could MT-1F and MT-3 be detected. Transcripts of MT-4 were detected in glycophorin A(+) and CD71(+) cells. Erythropoietin-responsive constitutive transcription of MT-1X and possible interleukin-3-responsive downregulation of MT-2A in ex vivo expanded precursors reveal their effect on MT biosynthesis. Biosynthesis and induction of MT at the protein level in the RBC precursors was also demonstrated by immunoblotting.