µTAS, Date: 2015/10/25 - 2015/10/29, Location: Gyeongju, Korea
Publication date:
2015-10-25
Pages:
849 -
851
ISSN:
9780979806483
Proceedings of the 19th International Conference on Miniaturized systems for Chemistry and Life Sciences
Author:
Pérez Ruiz, Elena
Decrop, Deborah ; De Vos, Ann ; Vanmechelen, Eugeen ; Kokalj, Tadej ; Spasic, Dragana ; Winderickx, Joris ; Lammertyn, Jeroen
Abstract:
© 15CBMS-0001. This manuscript reports a digital ELISA integrated on a digital microfluidics (DMF) platform for the ultrasensitive detection of Alzheimer's disease (AD) biomarker Tau protein over a wide range of relevant physiological concentrations. High-affinity antibodies combined with optimal assay conditions led to astonishing limits of detection in the low-attomolar range in buffer samples, which constitutes the first step toward developing a blood test for AD diagnosis.