Author:

Keywords:

performance liquid-chromatography, electrochemical detection, metabolites, neurotransmitters, catecholamines, modulation, serotonin, injection, dopamine, Science & Technology, Life Sciences & Biomedicine, Physical Sciences, Biochemical Research Methods, Chemistry, Analytical, Biochemistry & Molecular Biology, Chemistry, PERFORMANCE LIQUID-CHROMATOGRAPHY, ELECTROCHEMICAL DETECTION, METABOLITES, NEUROTRANSMITTERS, CATECHOLAMINES, MODULATION, SEROTONIN, INJECTION, DOPAMINE, 0301 Analytical Chemistry, 0399 Other Chemical Sciences, Analytical Chemistry, 3401 Analytical chemistry

Abstract:

Removal of retinal input from a restricted region of adult visual cortex leads to a substantial reorganisation of the retinotopy within the deprived zone. To investigate the role of the total (intra- and extracellular) concentration of the monoaminergic neuromodulators in the sub-cortical region of cat visual cortex, which is possibly involved in this reorganisation mechanism, a method for identification and quantification of noradrenaline, dopamine, serotonin, and their major metabolites, 3,4-dihydroxyphenylacetic acid, 4-hydroxy-3-methoxyphenylacetic acid, 5-hydroxyindole-3-acetic acid in small amounts of brain extracts has been developed and validated. Control or retinal lesion cats were killed with pentobarbital; the brains were quickly frozen, and 200 mum cryostat sections were cut.