Author:

Keywords:

Anti-sperm antibodies, CHROMATIN-STRUCTURE ASSAY, COVID-19, DNA fragmentation Index (DFI), DNA INTEGRITY, Fertility, General & Internal Medicine, IN-VITRO FERTILIZATION, Life Sciences & Biomedicine, Medicine, General & Internal, Medicine, Research & Experimental, Research & Experimental Medicine, Science & Technology, SEMEN, Sperm chromatin structure assay (SCSA), Sperm DNA damage, Humans, Follow-Up Studies, Semen Analysis, Prospective Studies, Chromatin, SARS-CoV-2, Longitudinal Studies, Immunoglobulin A, Immunoglobulin G, DNA Fragmentation, Semen, 1103 Clinical Sciences, 1117 Public Health and Health Services, 3202 Clinical sciences, 4202 Epidemiology

Abstract:

BACKGROUND: Short- and long-term implications of SARS-CoV-2 on the quality of the sperm and the results of this on fertility remain largely unknown due to lack of longitudinal studies. In this longitudinal observational cohort study, we aimed to analyse the differential effect and the impact of SARS-CoV-2 infection on different semen quality parameters. METHODS: Sperm quality was assessed using the World Health Organization criteria, DNA damage to sperm cells by quantifying the DNA fragmentation index (DFI) and the high-density stainability (HDS), IgA- and IgG-anti-sperm antibodies (ASA) were assessed with light microscopy. FINDINGS: SARS-CoV-2 infection was associated with sperm parameters that were independent of spermatogenic cycle like progressive motility, morphology, DFI and HDS, as well as spermatogenic cycle dependent parameters such as sperm concentration. Detection of IgA- and IgG-ASA allowed classification of patients in three different groups according to its sequence of appearance in sperm during post-COVID-19 follow-up. The maximum progressive motility was lowest during follow-up in patients without ASA (41.9%), intermediate in patients with only IgA-ASA (46.2%) and highest inpatients who had both IgA- and IgG-ASA (54.9%). INTERPRETATION: SARS-CoV-2 infection was associated with changes of all analysed sperm parameters to a different degree which is also observed in their return to normality and is suggestive of individual variations in the patient's immune system performance. Firstly, sperm production is decreased through temporal immune mediated arrest of active meiosis, and secondly immune induced sperm DNA damage prevents fertilization if transferred to the oocyte. Both mechanisms are temporal, and most sperm parameters return to baseline after infection. FUNDING: AML (R20-014), Femicare.