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Title: In vitro polarized transport of L-phenylalanine in human nasal epithelium and partial characterization of the amino acid transporters involved
Authors: Agu, Remigius ×
Dang, Hoang Vu
Jorissen, Mark
Willems, Tom
Vandoninck, Sandy
Van Lint, Johan
Vandenheede, Jackie
Kinget, Renaat
Verbeke, Norbert #
Issue Date: Aug-2003
Publisher: Thieme
Series Title: Pharmaceutical Research vol:20 issue:8 pages:1125-1132
Abstract: PURPOSE: The purpose of this study was to provide functional and molecular evidence to support the existence of large neutral amino acid transporters in human nasal epithelium using nasal primary cell culture model. METHODS: L-Phenylalanine was used as a model substrate to characterize carrier-mediated permeation of amino acids across human nasal epithelium. The influence of temperature, concentration, other amino acids, metabolic/transport inhibitors, and polarity/stereo-selectivity on transport of the model compound was investigated. Reverse transcriptase polymerase chain reaction was used for molecular characterization of the existence of the transporters. RESULTS: The transport of L-phenylalanine across the human nasal epithelium was polarized (apical --> basolateral >> basolateral --> apical), saturable (Km = 1.23 mM; Vmax = 805.1 nmol/mg protein/min) and stereo-selective (permeation of L-phenylalanine >> D-Phenylalanine). Its permeation was significantly (< 0.05) reduced by cationic, small and large neutral amino acids, oubain, amiloride, sodium-free medium, and temperature lowering. Reverse transcriptase polymerase chain reaction revealed the presence of the broad-scope cationic-dependent amino acid transporter gene (y+LAT-2) in the human nasal epithelium. CONCLUSIONS: Based on the results of this study, one may postulate that the human nasal epithelium expresses L-amino acid transporters. More studies are necessary for detailed characterization of the transporters.
URI: 
ISSN: 0724-8741
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Drug Delivery and Disposition
Research Group Experimental Oto-rhino-laryngology
Biochemistry Section (Medicine) (-)
Laboratory of Protein Phosphorylation and Proteomics
× corresponding author
# (joint) last author

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