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Title: Identification of MYPT1 and NIPP1 as subunits of protein phosphatase 1 in rat liver cytosol
Authors: Boudrez, An ×
Evens, K
Beullens, Monique
Waelkens, Etienne
Stalmans, Willy
Bollen, Mathieu #
Issue Date: Aug-1999
Publisher: Elsevier on behalf of the Federation of European Biochemical Societies
Series Title: FEBS Letters vol:455 issue:1-2 pages:175-8
Abstract: Various studies have provided evidence for the existence of spontaneously active cytosolic species of protein phosphatase 1, but these enzymes have never been purified and characterized. We have used chromatography on microcystin-Sepharose and Resource Q to purify cytosolic protein phosphatases from rat liver. Two of the isolated enzymes were identified by Western analysis and peptide sequencing as complexes of the catalytic subunit of protein phosphatase 1 and either the inhibitor NIPP1 or the myosin-binding subunit MYPT1, which reportedly is not present in chicken liver. In contrast, PCR cloning revealed the expression of two MYPT1 splice variants in rat liver.
ISSN: 0014-5793
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry Section (Medicine) (-)
Laboratory of Protein Phosphorylation and Proteomics
Department of Cellular and Molecular Medicine - miscellaneous
Laboratory of Biosignaling & Therapeutics
Laboratory of Phosphoproteomics (-)
× corresponding author
# (joint) last author

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