Title: Inhibition of inositol trisphosphate-induced calcium release by caffeine is prevented by ATP
Authors: Missiaen, Ludwig ×
Parys, Jan
De Smedt, Humbert
Himpens, Bernard
Casteels, Rik #
Issue Date: Jun-1994
Series Title: The Biochemical journal. vol:300 ( Pt 1) pages:81-4
Abstract: We have investigated the effect of various methylxanthines on the basal and InsP3-stimulated unidirectional 45Ca2+ efflux from permeabilized A7r5 cells under different experimental conditions. We report that caffeine and theophylline inhibit the InsP3-induced Ca2+ release, whereas the basal Ca2+ leak remained largely unaffected. The effect on InsP3-induced Ca2+ release was specific, since isocaffeine was not effective. The inhibition occurred similarly in the absence or presence of extravesicular Ca2+ and was not associated with a decrease in the [3H]InsP3 binding to the receptor. ATP and MgATP (5 mM) prevented the inhibition, suggesting that caffeine may interact with an ATP-binding site on the InsP3 receptor or some associated protein.
ISSN: 0264-6021
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Physiology Section (-)
Pharmacology Section (-)
Laboratory of Molecular and Cellular Signaling
× corresponding author
# (joint) last author

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