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Keywords:

Amino Acid Sequence, Bacterial Proteins, Base Sequence, Carboxyl and Carbamoyl Transferases, Chromosome Mapping, DNA, Bacterial, Fabaceae, Genes, Bacterial, Genetic Complementation Test, Lipopolysaccharides, Methyltransferases, Molecular Sequence Data, Mutation, N-Acetylglucosaminyltransferases, Plants, Medicinal, Research Support, Non-U.S. Gov't, Rhizobium, Sequence Homology, Amino Acid, Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, Biotechnology & Applied Microbiology, Plant Sciences, HOST RANGE, NOD FACTOR, AZORHIZOBIUM-CAULINODANS, N-METHYL, SP-NOV, NGR234, IDENTIFICATION, PLASMID, VECTORS, 0604 Genetics, 0605 Microbiology, 0607 Plant Biology, Plant Biology & Botany, 3105 Genetics, 3107 Microbiology, 3108 Plant biology

Abstract:

Rhizobium tropici strain CIAT899 induces nitrogen-fixing nodules on the roots of a wide range of tropical legumes, including Phaseolus vulgaris and Leucaena leucocephala. Previously, a DNA region of the CIAT899 pSym plasmid containing the common nodulation genes nodABC and one of the nodD alleles was characterized (P. van Rhijn, B. Feys, C. Verreth, and J. Vanderleyden, J. Bacteriol. 175: 438-447, 1993). As reported here, the region immediately downstream of nodC contains the nodSU genes. The nucleotide sequence of these genes is presented. CIAT899 nodS and nodU mutants were constructed. The nodS mutant was completely deficient in nodulation on the host plants P. vulgaris and L. leucocephala. The nodU mutation caused a decrease in nodulation on Leucaena but resorted no effect on Phaseolus. Introduction of the CIAT899 nodABCSU region in R. etli CE-3, a strain that only nodulates P. vulgaris, caused an extension of the host range of strain CE-3 to L. leucocephala.