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Title: Nuclear and subnuclear targeting sequences of the protein phosphatase-1 regulator NIPP1
Authors: Jagiello, Izabela ×
Van Eynde, Aleyde
Vulsteke, Veerle
Beullens, Monique
Boudrez, An
Keppens, Stefaan
Stalmans, Willy
Bollen, Mathieu #
Issue Date: Nov-2000
Publisher: Co. of Biologists
Series Title: Journal of Cell Science vol:113 Pt 21 pages:3761-8
Abstract: NIPP1 is a nuclear subunit of protein phosphatase-1 (PP1) that colocalizes with pre-mRNA splicing factors in speckles. We report here that the nuclear and subnuclear targeting of NIPP1, when expressed in HeLa cells or COS-1 cells as a fusion protein with the enhanced-green-fluorescent protein (EGFP), are mediated by distinct sequences. While NIPP1-EGFP can cross the nuclear membrane passively, the active transport to the nucleus is mediated by two independent nuclear localization signals in the central domain of NIPP1, which partially overlap with binding site(s) for PP1. Furthermore, the concentration of NIPP1-EGFP in the nuclear speckles requires the 'ForkHead-Associated' domain in the N terminus. This domain is also required for the nuclear retention of NIPP1 when active transport is blocked. Our data imply that the nuclear and subnuclear targeting of NIPP1 are controlled independently.
URI: 
ISSN: 0021-9533
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry Section (Medicine) (-)
Molecular Genetics Section (-)
Department of Cellular and Molecular Medicine - miscellaneous
Laboratory of Biosignaling & Therapeutics
× corresponding author
# (joint) last author

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