Title: Myosin light chain phosphorylation-dependent modulation of volume-regulated anion channels in macrovascular endothelium
Authors: Nilius, Bernd ×
Prenen, Jean
Walsh, M P
Carton, Iris
Bollen, Mathieu
Droogmans, Guillaume
Eggermont, Jan #
Issue Date: Mar-2000
Publisher: Elsevier on behalf of the Federation of European Biochemical Societies
Series Title: FEBS Letters vol:466 issue:2-3 pages:346-50
Abstract: The Rho/Rho-associated kinase (ROK) pathway has been shown to modulate volume-regulated anion channels (VRAC) in cultured calf pulmonary artery endothelial (CPAE) cells. Since Rho/ROK can increase myosin light chain phosphorylation, we have now studied the effects of inhibitors of myosin light chain kinase (MLCK) or myosin light chain phosphatase (MLCP) on VRAC in CPAE. Application of ML-9, an MLCK inhibitor, inhibited VRAC, both when applied extracellularly or when dialyzed into the cell. A similar inhibitory effect was obtained by dialyzing the cells with AV25, a specific MLCK inhibitory peptide. Conversely, NIPP1(191-210), an MLCP inhibitory peptide, potentiated the activation of VRAC by a 25% hypotonic stimulus. These data indicate that activation of VRAC is modulated by MLC phosphorylation.
ISSN: 0014-5793
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Physiology Section (-)
Biochemistry Section (Medicine) (-)
Laboratory of Ion Channel Research
Department of Cellular and Molecular Medicine - miscellaneous
Laboratory of Cellular Transport Systems
Laboratory of Biosignaling & Therapeutics
× corresponding author
# (joint) last author

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