Title: Simultaneous recording of Indo-1 fluorescence and Na+/Ca2+ exchange current reveals two components of Ca2(+)-release from sarcoplasmic reticulum of cardiac atrial myocytes
Authors: Lipp, P ×
Pott, L
Callewaert, Geert
Carmeliet, Edward #
Issue Date: Feb-1991
Series Title: FEBS Letters vol:275 issue:1-2 pages:181-4
Abstract: Simultaneous measurements of intracellular Ca2(+)-concentration ([Ca2+]i) using Indo-1 and the current generated by electrogenic Na+/Ca2(+)-exchange (INaCa) have been performed on atrial myocytes from hearts of adult guinea-pigs. Whereas the fluorescence-measurements provide information on global [Ca2+]i, InaCa which is a linear function of Ca2(+)-concentration, indicates subsarcolemmal [Ca2+]. Under conditions in which intracellular Ca2(+)-transients due to Ca2(+)-release from the sarcoplasmic reticulum (SR) have been artificially slowed, a deviation between the two different Ca2(+)-signals can be found. During onset of release signals Ca2(+)-concentration seen by the membrane is higher than global [Ca2+]i. Our results provide evidence that in atrial myocytes, lacking a T-system, Ca2(+)-induced Ca2(+)-release occurs first from a subsarcolemmal compartment of the SR. The resulting Ca2(+)-transient serves to trigger Ca2(+)-release from deeper SR-compartments.
ISSN: 0014-5793
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Physiology Section (-)
× corresponding author
# (joint) last author

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