Transcriptome analysis during cell division in plants
Breyne, Peter Dreesen, Rozemarijn Vandepoele, Klaas De Veylder, Lieven Van Breusegem, Frank Callewaert, Lindy Rombauts, Stephane Raes, J Cannoot, Bernard Engler, Gilbert Inzé, Dirk Zabeau, Marc #
Proceedings of the National Academy of Sciences of the United States of America vol:99 issue:23 pages:14825-30
Using synchronized tobacco Bright Yellow-2 cells and cDNA-amplified fragment length polymorphism-based genomewide expression analysis, we built a comprehensive collection of plant cell cycle-modulated genes. Approximately 1,340 periodically expressed genes were identified, including known cell cycle control genes as well as numerous unique candidate regulatory genes. A number of plant-specific genes were found to be cell cycle modulated. Other transcript tags were derived from unknown plant genes showing homology to cell cycle-regulatory genes of other organisms. Many of the genes encode novel or uncharacterized proteins, indicating that several processes underlying cell division are still largely unknown.