Author:

Keywords:

capsular polysaccharides, pneumococcal surface protein a, costimulatory molecule, pneumococcal capsular polysaccharides, cd4(+)cd25(+) t-cells, immune-responses, in-vivo, monoclonal-antibodies, isotype responses, 4-1bb, mice, lymphocytes, antigens, Science & Technology, Life Sciences & Biomedicine, Cell Biology, Hematology, Immunology, pneumococcal surface protein A, PNEUMOCOCCAL CAPSULAR POLYSACCHARIDES, CD4(+)CD25(+) T-CELLS, IMMUNE-RESPONSES, IN-VIVO, MONOCLONAL-ANTIBODIES, ISOTYPE RESPONSES, 4-1BB, MICE, LYMPHOCYTES, ANTIGENS, 4-1BB Ligand, Animals, Antibodies, Bacterial, Antibodies, Monoclonal, Antibody Formation, Antigens, Bacterial, Bacterial Capsules, Bacterial Proteins, Gene Expression Regulation, Immunization, Immunoglobulin G, Immunoglobulin M, Ligands, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Pneumococcal Infections, Pneumococcal Vaccines, Polysaccharides, Bacterial, Streptococcus pneumoniae, Tumor Necrosis Factor Receptor Superfamily, Member 9, 0601 Biochemistry and Cell Biology, 1107 Immunology, 3204 Immunology

Abstract:

Protection against infection with Streptococcus pneumoniae is based mainly on the generation of antibodies to the pneumococcal capsular polysaccharides (caps-PS). Although caps-PS are considered thymus-independent antigens, there is a growing body of evidence that T lymphocytes and costimulatory molecules are involved in the regulation of the antibody response to caps-PS. We investigated whether the interaction between 4-1BB and 4-1BB ligand (4-1BBL) is involved in the modulation of the antibody response to caps-PS after immunization with Pneumovax((R)) or with intact heat-killed S. pneumoniae. Treatment with agonistic anti-4-1BB mAb, which mimics engagement of 4-1BB by 4-1BBL, had no effect on the IgG and IgM immune response to caps-PS (Serotype 3) after immunization with Pneumovax or with S. pneumoniae Serotype 3. However, anti-4-1BB treatment strongly inhibited the IgG response to pneumococcal surface protein A ( PspA). By contrast, the IgG anti-caps-PS (Serotype 3) antibody response was reduced strongly in 4-1BBL(-/-) mice immunized with S. pneumoniae Serotype 3. The IgG anti-PspA antibody response in the 4-1BB(-/-) mice was comparable with the immune response in the wild-type mice. We conclude that distinct pathways are involved in the humoral antibody response to pneumococcal antigens, depending on the nature of the antigen and the context in which the different antigens are presented. The 4-1BB-4-1BBL interaction is not involved in the antibody response to soluble capsPS. The influence of the 4-1BB-4-1BBL interaction in the immune reaction to S. pneumoniae Serotype 3 depends on the experimental system used.