Title: Structure-based assembly of protein complexes in yeast
Authors: Aloy, Patrick ×
Böttcher, Bettina
Ceulemans, Hugo
Leutwein, Christina
Mellwig, Christian
Fischer, Susanne
Gavin, Anne-Claude
Bork, Peer
Superti-Furga, Giulio
Serrano, Luis
Russell, Robert B #
Issue Date: Mar-2004
Series Title: Science. vol:303 issue:5666 pages:2026-9
Abstract: Images of entire cells are preceding atomic structures of the separate molecular machines that they contain. The resulting gap in knowledge can be partly bridged by protein-protein interactions, bioinformatics, and electron microscopy. Here we use interactions of known three-dimensional structure to model a large set of yeast complexes, which we also screen by electron microscopy. For 54 of 102 complexes, we obtain at least partial models of interacting subunits. For 29, including the exosome, the chaperonin containing TCP-1, a 3'-messenger RNA degradation complex, and RNA polymerase II, the process suggests atomic details not easily seen by homology, involving the combination of two or more known structures. We also consider interactions between complexes (cross-talk) and use these to construct a structure-based network of molecular machines in the cell.
ISSN: 0036-8075
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry Section (Medicine) (-)
× corresponding author
# (joint) last author

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