Title: Characterisation of human peroxisomal 2,4-dienoyl-CoA reductase
Authors: De Nys, Katelijne
Meyhi, Els
Mannaerts, Guy
Fransen, Marc
Van Veldhoven, Paul P # ×
Issue Date: Aug-2001
Publisher: Elsevier Science
Series Title: Biochimica et Biophysica Acta. Molecular and Cell Biology of Lipids vol:1533 issue:1 pages:66-72
Abstract: Based on the primary structure of the rat peroxisomal 2,4-dienoyl-CoA reductase (M. Fransen, P.P. Van Veldhoven, S. Subramani, Biochem. J. 340 (1999) 561-568), the cDNA of the human counterpart was cloned. It contained an open reading frame of 878 bases encoding a protein of 291 amino acids (calculated molecular mass 30778 Da), being 83% identical to the rat reductase. The gene, encompassing nine exons, is located at chromosome 16p13. Bacterially expressed poly(His)-tagged reductase was active not only towards short and medium chain 2,4-dienoyl-CoAs, but also towards 2,4,7,10,13,16,19-docosaheptaenoyl-CoA. Hence, the reductase does not seem to constitute a rate limiting step in the peroxisomal degradation of docosahexaenoic acid. The reduction of docosaheptaenoyl-CoA, however, was severely decreased in the presence of albumin.
ISSN: 1388-1981
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmacology Section (-)
Department of Pharmaceutical & Pharmacological Sciences - miscellaneous
Laboratory of Lipid Biochemistry and Protein Interactions
× corresponding author
# (joint) last author

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