Title: Determinants of the nucleolar targeting of protein phosphatase-1
Authors: Lesage, Bart ×
Beullens, Monique
Ceulemans, Hugo
Himpens, Bernard
Bollen, Mathieu #
Issue Date: Oct-2005
Publisher: Elsevier on behalf of the Federation of European Biochemical Societies
Series Title: FEBS Letters vol:579 issue:25 pages:5626-30
Abstract: The ubiquitously expressed protein Ser/Thr phosphatase-1 isoforms PP1alpha, PP1beta and PP1gamma1 are dynamically targeted to distinct, but overlapping cellular compartments by associated proteins. Within the nucleus of HeLa cells, EGFP-tagged PP1gamma1 and PP1beta were predominantly targeted to the nucleoli, while PP1alpha showed a more diffuse distribution. Using PP1 chimaeras and point mutants we show here that a single N-terminal residue, i.e., Gln20 for PP1alpha, Arg19 for PP1beta and Arg20 for PP1gamma1 accounts for their distinct subnuclear distribution. Our data also suggest that the N-terminus of PP1beta and PP1gamma1 harbours an interaction site for one or more nucleolar interactors.
ISSN: 0014-5793
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry Section (Medicine) (-)
Laboratory of Biosignaling & Therapeutics
Laboratory of Molecular and Cellular Signaling
× corresponding author
# (joint) last author

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