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Title: Long-lasting changes in GABA responsiveness in cultured neurons
Authors: Chabwine, Joelle N ×
Van Damme, Philip
Eggermont, Jan
De Smedt, Humbert
Missiaen, Ludwig
Van Den Bosch, Ludo
Parys, Jan
Robberecht, Wim
Callewaert, Geert #
Issue Date: Jul-2004
Series Title: Neuroscience Letters vol:365 issue:1 pages:69-72
Abstract: In neuronal cells, GABA evokes an increase in chloride conductance by activating GABA(A) and GABA(C) receptors. In mature neurons, this increase in conductance generally has a hyperpolarizing and inhibitory action. Using gramicidin-based perforated patch recordings, we show that in cultured motor neurons GABA-induced currents are significantly altered following activation of GABA receptors coinciding with changes in membrane potential. Changes in intracellular chloride concentration constituted the mechanism for this modulation. Because of low resting chloride conductance and low activity of chloride transporters, changes in intracellular chloride concentration and hence GABA response were long-lasting (time constant of recovery was about 2.5 min). Cultured dorsal horn and hippocampal neurons exhibited a similar response pattern, suggesting a general property of cultured neuronal cells. These long-lasting changes in GABA responsiveness may have major implications on neuronal excitability.
URI: 
ISSN: 0304-3940
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Physiology Section (-)
Research Group Experimental Neurology
Laboratory of Molecular and Cellular Signaling
Laboratory for Neurobiology (Vesalius Research Center)
Laboratory of Cellular Transport Systems
× corresponding author
# (joint) last author

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