Title: Binding of IRBIT to the IP3 receptor: determinants and functional effects
Authors: Devogelaere, Benoit ×
Nadif Kasri, Nael
Derua, Rita
Waelkens, Etienne
Callewaert, Geert
Missiaen, Ludwig
Parys, Jan
De Smedt, Humbert #
Issue Date: Apr-2006
Publisher: Academic Press
Series Title: Biochemical and Biophysical Research Communications vol:343 issue:1 pages:49-56
Abstract: IRBIT has previously been shown to interact with the inositol 1,4,5-trisphosphate (IP3) receptor (IP3R) in an IP3-sensitive way. So far it remained to be elucidated whether this interaction was direct or indirect, and whether it was functionally relevant. We now show that IRBIT can directly interact with the IP3R, and that both the suppressor domain and the IP3-binding core of the IP3R are essential for a strong interaction. Moreover, we identified a PEST motif and a PDZ-ligand on IRBIT which were critical for the interaction with the IP3R. Furthermore, we identified Asp-73 as a critical residue for this interaction. Finally, we demonstrated that this interaction functionally affects the IP3R: IRBIT inhibits both IP3 binding and IP3-induced Ca2+ release.
ISSN: 0006-291X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Physiology Section (-)
Laboratory of Molecular and Cellular Signaling
Laboratory of Protein Phosphorylation and Proteomics
Laboratory of Phosphoproteomics (-)
× corresponding author
# (joint) last author

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