Title: Critical evaluation of cytosolic calcium determination in resting muscle fibres from normal and dystrophic (mdx) mice
Authors: Gailly, P ×
Boland, B
Himpens, Bernard
Casteels, Rik
Gillis, J M #
Issue Date: Sep-1993
Publisher: Elsevier
Series Title: Cell Calcium vol:14 issue:6 pages:473-483
Abstract: The fluorescent probe Fura-2/AM was used to determine cytosolic free calcium concentration in soleus muscle and in isolated flexor digitorum brevis fibres. This required a precise calibration; therefore, each calibration parameter was studied in situ. The influence of the dye concentration on calcium measurements was also examined. This precise calibration technique was used to compare absolute free calcium concentration in resting preparations from dystrophic (mdx) and control (C57) mice. We showed that the behavior of the dye was not similar in C57 and in mdx muscles. For this reason, we did not confirm the previous results that cytosolic free calcium concentration is increased in mdx muscles.
ISSN: 0143-4160
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Physiology Section (-)
Laboratory of Molecular and Cellular Signaling
× corresponding author
# (joint) last author

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