Title: Simplified strategy for detection of recombinant human immunodeficiency virus type 1 group M isolates by gag/env heteroduplex mobility assay
Authors: Heyndrickx, L ×
Janssens, W
Zekeng, L
Musonda, R
Anagonou, S
Van der Auwera, G
Coppens, Steve
Vereecken, Karen
De Witte, K
Van Rampelbergh, R
Kahindo, M
Morison, L
McCutchan, FE
Carr, JK
Albert, J
Essex, M
Goudsmit, J
Asjo, B
Salminen, M
Buve, A
van der Groen, G #
Issue Date: Jan-2000
Publisher: Amer soc microbiology
Series Title: Journal of Virology vol:74 issue:1 pages:363-370
Abstract: We developed a heteroduplex mobility assay in the gag gene (gag HMA) for the identification of group M subtypes A to H. The assay covers the region coding for amino acid 132 of p24 to amino acid 20 of p7 (according to human immunodeficiency virus type 1 [HIV-1] ELI, 460 bp). The gag HMA was compared with sequencing and phylogenetic analysis of an evaluation panel of 79 HIV-1 group M isolates isolated from infected individuals from different geographic regions. Application of gag HMA in combination with env HMA on 252 HIV-1-positive plasma samples from Benin, Cameroon, Kenya, and Zambia revealed a high prevalence of a variety of intersubtype recombinants in Yaounde, Cameroon (53.8%); Kisumu, Kenya (26.8%); and Cotonou, Benin (41%); no recombinants were identified among the samples from Ndola, Zambia. The AG(IbNG) circulating recombinant form, as determined by gag HMA, was found to be the most common intersubtype recombinant in Yaounde (39.4%) and Cotonou (38.5%). Using a one-tube reverse transcriptase PCR protocol, this gag HMA in combination with env HMA is a useful tool for rapidly monitoring the prevalence of the various genetic subtypes as well as of recombinants of HIV-1. Moreover, this technology can easily be applied in laboratories in developing countries.
ISSN: 0022-538X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Clinical Residents Medicine
Chemical Engineering - miscellaneous
× corresponding author
# (joint) last author

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