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Title: Genomic organisation, chromosomal localisation tissue distribution and developmental regulation of the PR61/B' regulatory subunits of protein phosphatase 2A in mice
Authors: Martens, Ellen ×
Stevens, Ilse
Janssens, Veerle
Vermeesch, Joris
Götz, Jürgen
Goris, Jozef
Van Hoof, Christine #
Issue Date: Apr-2004
Series Title: Journal of Molecular Biology vol:336 issue:4 pages:971-86
Abstract: Protein phosphatase 2A (PP2A) is a major serine/threonine-specific phosphatase playing central roles in development, cell growth and transformation. Regulation is largely accomplished by the regulatory B subunits, which determine substrate specificity, subcellular localisation and catalytic activity. The B' family, also known as the PR61 family, is the most diverse, consisting of five genes (alpha,beta,gamma,delta and epsilon) that give rise to a number of splice variants. We deduced the sequences of the different PR61 proteins in mice and found evidence for the expression of PR61alpha, beta1, gamma1, gamma2, gamma3, delta1 and epsilon. We report the genomic organisation and localisation of the murine PR61 genes (Ppp2r5a-Ppp2r5e). This information will be useful for the future realisation of PR61 knockouts. Using Northern blotting, we examined the expression of the five PR61 isoforms in different tissues. A brain-specific function can be expected for the PR611beta protein based on the high expression levels observed in murine brain. In situ hybridisation analysis of the adult brain revealed a distinct and partially overlapping pattern of mRNA expression of the various PR61 isoforms. The PR61 mRNA expression during embryonic development was examined by Northern blotting. The PR61 transcripts were differentially expressed, suggesting a specific function for each of the PR61 proteins during embryonic development and/or adult life.
ISSN: 0022-2836
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry Section (Medicine) (-)
Department of Materials Engineering - miscellaneous
Clinical Genetics Section (-)
Faculty of Medicine - miscellaneous
Laboratory of Protein Phosphorylation and Proteomics
Faculty of Pharmaceutical Sciences - miscellaneous
Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

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