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Title: Ciliogenesis and coordinated ciliary beating in human nasal epithelial cells cultured in vitro
Authors: Jorissen, Mark
Van der Schueren, Bernadette
Tyberghein, Jacques
Van den Berghe, Herman
Cassiman, Jean-Jacques #
Issue Date: Nov-1989
Series Title: Acta Oto-Rhino-Laryngologica Belgica vol:43 issue:1 pages:67-73
Abstract: Human nasal epithelial cells, dissociated from nasal polyps, lost all their cilia when cultured as monolayers on 0.2% collagen gels in Ham's F12-DME 1/1, supplemented with NU-serum 10%, choleratoxin (10 ng/ml), retinoic acid (10(7) M) and antibiotics. These deciliated epithelial cell sheaths were then placed in a suspension culture system, and epithelial aggregates and vesicles formed. After 1 week in suspension, cilia progressively reappeared. This in vitro ciliogenesis resulted in ultrastructurally normal cilia with an intercellularly coordinated beating pattern. These epithelial aggregates and vesicles maintained this differentiated ciliary morphology and function for more than 6 months.
ISSN: 0001-6497
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Human Mutations and Polymorphisms Section (-)
Research Group Experimental Oto-rhino-laryngology
Clinical Genetics Section (-)
Forensic Biomedical Sciences
# (joint) last author

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