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Title: Targeted disruption of the murine Plag1 proto-oncogene causes growth retardation and reduced fertility
Authors: Hensen, Karen ×
Braem, Caroline
Declercq, Jeroen
Van Dyck, Frederik
Dewerchin, Mieke
Fiette, Laurence
Denef, Carl
Van de Ven, Willem #
Issue Date: Dec-2004
Publisher: Japanese Society of Developmental Biologists
Series Title: Development, growth & differentiation vol:46 issue:5 pages:459-70
Abstract: The pleomorphic adenoma gene 1 (Plag1) proto-oncogene encodes a transcription factor and is implicated in human tumorigenesis via ectopic overexpression. No information is available about its developmental role. To address this, a Plag1-/- mouse strain was generated and it appears that Plag1-deficient mice are viable. No anatomical differences are obvious at birth, except that the weight of Plag1-/- mice is significantly lower in comparison to control litter mates. This early growth retardation is maintained throughout adult life with proportionally smaller organs except for the disproportionally small seminal vesicles and ventral prostate; however, plasma testosterone levels in males were not affected. Furthermore, fertility of both male and female Plag1-/- is reduced. Northern blot analysis revealed that Plag1 is developmentally regulated with high overall fetal expression levels, which drop after birth. Furthermore, Plag1 is differentially expressed and is readily detectable in the reproductive organs and pituitary. Expression of growth regulatory Igf2, a known target gene of Plag1 in tumorigenesis, was not affected in Plag1-/- embryos and pups. The general morphology and histology of the size-reduced pituitaries was not affected. Our results establish that Plag1 disruption in mouse differentially affects pre- and postnatal growth and development of organs, with reproductive repercussions.
URI: 
ISSN: 0012-1592
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Human Genetics - miscellaneous
Pharmacology Section (-)
Physiology Section (-)
Associated Laboratories - miscellaneous (-)
Interdepartemental Stem Cell Institute (-)
Molecular and Vascular Biology
Laboratory for Biochemical Neuroendocrinology
Laboratory of Angiogenesis and Vascular Metabolism (Vesalius Research Center) (+)
× corresponding author
# (joint) last author

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