Title: Representational difference analysis using minute quantities of DNA
Authors: Michiels, L ×
Van Leuven, Freddy
van den Oord, Joost
de Wolf-Peeters, C
Delabie, Jan #
Issue Date: Sep-1998
Publisher: Oxford University Press
Series Title: Nucleic Acids Research vol:26 issue:15 pages:3608-3610
Abstract: Representational difference analysis (RDA) is a differential hybridization method which can effectively isolate unique DNA sequences from complex and highly related genomes or cDNA libraries. A major drawback of the RDA analysis is the requirement for pure driver and relatively pure tester samples, ruling out the analysis of whole tissue biopsies. To circumvent this problem, we have modified the technique for the analysis of very small quantities of DNA so that pure cell populations isolated by micromanipulation from tissue sections can be analyzed. Using this modified technique, as few as 50 diploid cells ( approximately 500 pg of DNA) can be analyzed.
ISSN: 0305-1048
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Translational Cell & Tissue Research
Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science