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Title: Stable expression of VLA-4 and increased maturation of the beta 1-integrin precursor after transfection of CHO cells with alpha 4m cDNA
Authors: Jaspers, M
De Meirsman, Catherine
Schollen, E
Vekemans, Sylvie
Cassiman, Jean-Jacques #
Issue Date: Nov-1994
Series Title: FEBS Letters vol:353 issue:3 pages:239-42
Abstract: A full-length cDNA coding for the murine alpha 4 integrin subunit (alpha 4m) was transfected into CHO-K1 cells and cell lines that expressed VLA-4 at their surface as a result of the association of transfected alpha 4m with endogenous hamster beta 1 were selected. Functionality of the expressed alpha 4m beta 1 was shown by adhesion assays on VCAM-1 and antibody (anti-VCAM-1) inhibition. Pulse chase experiments indicated that transfection of the murine alpha 4 cDNA into CHO cells led to an increase in maturation and a decrease in degradation of the beta 1 precursor subunit compared to control CHO-K1 cells. This was supported by FACS analysis, using an anti-hamster beta 1 monoclonal antibody, which showed that more beta 1 subunit was expressed at the surface of these stably transfected alpha 4m expressing cells. These results support the hypothesis that degradation of precursor beta 1 is at least partly determined by the quantity of alpha subunits available intracellulary for heterodimer formation.
ISSN: 0014-5793
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Human Mutations and Polymorphisms Section (-)
Forensic Biomedical Sciences
Department of Health and Technology - UC Leuven
# (joint) last author

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