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Title: Coexpression of human cdk5 and its activator p35 with human protein tau in neurons in brain of triple transgenic mice
Authors: Van Den Haute, Chris ×
Spittaels, K
Van Dorpe, Jo
Lasrado, Reena
Vandezande, K
Laenen, Isabel
Geerts, H
Van Leuven, Freddy #
Issue Date: Feb-2001
Publisher: Blackwell Science
Series Title: Neurobiology of Disease vol:8 issue:1 pages:32-44
Abstract: The potential contribution of cyclin-dependent protein kinase 5 (cdk5) to hyperphosphorylate protein tau, as claimed in Alzheimer's disease, was investigated in vivo. We generated single, double, and triple transgenic mice that coexpress human cdk5 and its activator p35 as well as human protein tau in cerebral neurons. Whereas expression and increased cdk5-kinase activity was obtained, as measured in vitro and demonstrated in vivo, neither murine nor human protein tau was appreciably phosphorylated in the brain of double and triple transgenic mice. These mice behaved and reproduced normally. Silver impregnation and immunohistochemistry of brain sections demonstrated that neurofilament proteins became redistributed in apical dendrites of cortical neurons. This suggested a cytoskeletal effect, but no other relevant brain pathology became apparent. These observations indicate that cdk5/p35 is not a major protein tau kinase and that cdk5/p35 did not cause neurodegeneration in mouse brain, as opposed to cdk5/p25.
ISSN: 0969-9961
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Associated Laboratories - miscellaneous (-)
Research Group for Neurobiology and Gene Therapy
Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

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