Title: Hepatitis B virus replication causes oxidative stress in HepAD38 liver cells
Authors: Severi, Tamara ×
Ying, Chunxiao
Vermeesch, Joris
Cassiman, David
Cnops, Lieselotte
Verslype, Chris
Fevery, Johan
Arckens, Lut
Neyts, Johan
van Pelt, Jos #
Issue Date: Oct-2006
Publisher: Dr. W. Junk B.V. Publishers
Series Title: Molecular and Cellular Biochemistry vol:290 issue:1-2 pages:79-85
Abstract: We used human hepatoma HepAD38 cells, in which HBV production is under the control of a tetracycline-regulated promotor, to investigate changes induced in the host cell by HBV replication that could contribute to malignant transformation. Parameters of oxidative stress (malondialdehyde, glutathione) and cell proliferation were determined at different times after induction (0-96 h). In HBV-producing cells, the redox status peaked at 72 h. cDNA micro array analysis at 72 h post induction revealed 3 groups of genes that were up-regulated by HBV: (i) heat shock proteins, (ii) oxidative and metabolic stress and (iii) growth and apoptosis related genes. Continuous HBV production did not accelerate karyotypic changes in cells cultured for 4 months (18 passages). In conclusion: HBV replication modulates host gene expression and induces oxidative stress. In this HepAD38 model early events (0-4 days) in the host cell after induction of HBV replication can be studied under strictly defined conditions.
ISSN: 0300-8177
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Hepatology
Rega Institute for Medical Research (-)
Animal Physiology and Neurobiology Section - miscellaneous
Laboratory of Virology and Chemotherapy (Rega Institute)
Clinical Genetics Section (-)
Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

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