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Title: A physical map of the chromosome 12 centromere
Authors: Vermeesch, Joris ×
Duhamel, Hein
Raeymaekers, Petra
Van Zand, K
Verhasselt, Peter
Fryns, Jean-Pierre
Marynen, Peter #
Issue Date: Mar-2004
Publisher: S. Karger
Series Title: Cytogenetic and Genome Research vol:103 issue:1-2 pages:63-73
Abstract: While current sequencing efforts consider the detection of alpha satellite repeats as logical end points for map construction, detailed maps of most pericentromeric regions are lacking to confirm this hypothesis. Here we identify the different alpha satellite families present at the pericentromeric region of chromosome 12. The order, size and location of these repeats is established using radiation hybrid analysis, pulsed field gel analysis and FISH and the maps are integrated with current sequence information. For the different classes of alpha satellites present at the chromosome 12 centromere the paralogs in the human genome were mapped by FISH. Unique sequences flanking the alpha satellite repeats were identified, some of which are not represented in the current draft sequence. This mapping effort localises the different alpha satellite repeats within the pericentromeric region and anchors them in the current maps. The novel sequences identified may serve as the end point for the ongoing sequencing efforts.
ISSN: 1424-8581
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Translational Research in GastroIntestinal Disorders
Clinical Genetics Section (-)
Molecular Genetics Section (-)
Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

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