Title: Cell-Free HIV-1 Transcytosis Through Primary Genital Epithelial Cells
Authors: Bobardt, Michael D ×
Chatterji, Udayan
Selvarajah, Suganya
Van der Schueren, Bernadette
David, Guido
Kahn, Bruce
Gallay, Philippe A #
Issue Date: Jan-2007
Publisher: American Society for Microbiology (ASM)
Series Title: Journal of Virology vol:81 issue:1 pages:395-405
Abstract: Although the transport of HIV-1 through the epithelium is critical for HIV-1 colonization, the mechanisms controlling this process remain obscure. In the present study, we investigated the transcellular migration of HIV-1 as a cell-free virus through primary genital epithelial cells (PGECs). The absence of CD4 on PGECs implicates an unusual entry pathway for HIV-1. We found that syndecans are abundantly expressed on PGECs and promote the initial attachment and subsequent entry of HIV-1 through PGECs. Although CXCR4 and CCR5 do not contribute to HIV-1 attachment, they enhance viral entry and transcytosis through PGECs. Importantly, HIV-1 exploits both syndecans and chemokine receptors to ensure successful cell-free transport through the genital epithelium. HIV-1-syndecan interactions rely on specific residues in the V3 of gp120 and specific sulfations within syndecans. We found no obvious correlation between co-receptor usage and the capacity of the virus to transcytose. Since viruses isolated after sexual transmission are mainly R5 viruses, this suggests that the properties conferring virus replication after transmission are distinct from those conferring cell-free virus transcytosis through the genital epithelium. Although we found that cell-free HIV-1 crosses PGECs as infectious particles, the efficiency of transcytosis is extremely poor, less than 0.02% of the initial inoculum. This demonstrates that the genital epithelium serves as a major barrier against HIV-1. Although one cannot exclude the possibility that a low passage of cell-free HIV-1 transcytosis through an intact genital epithelium occurs in vivo, it is likely that the establishment of infection via cell-free HIV-1 transmigration is a rare event.
ISSN: 0022-538X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Genetics Section (-)
Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

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