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Title: [(18)F]FDG PET monitoring of tumour response to chemotherapy: does [(18)F]FDG uptake correlate with the viable tumour cell fraction?
Authors: Spaepen, Karoline ×
Stroobants, Sigrid
Dupont, Patrick
Bormans, Guy
Balzarini, Jan
Verhoef, Gregor
Mortelmans, Luc
Vandenberghe, Peter
De Wolf-Peeters, Christiane #
Issue Date: May-2003
Publisher: Springer-verlag
Series Title: European journal of nuclear medicine and molecular imaging. vol:30 issue:5 pages:682-8
Abstract: Because metabolic changes induced by chemotherapy precede the morphological changes, fluorine-18 fluorodeoxyglucose positron emission tomography ([(18)F]FDG PET) is thought to predict response to therapy earlier and more accurately than other modalities. To be a reliable predictor of response, changes in tumour [(18)F]FDG uptake should reflect changes in viable cell fraction, but little is known about the contribution of apoptotic and necrotic cancer cells and inflammatory tissue to the [(18)F]FDG signal. In a tumour mouse model we investigated the relation between chemotherapy-induced changes in various tumoral components and tumour uptake and size. SCID mice were subcutaneously inoculated in the right thigh with 5 x 10(6) Daudi cells. When the tumour measured 15-20 mm, Endoxan was given intravenously. At different time points [1-15 days (d1-d15) after the injection of Endoxan], ex vivo autoradiography and histopathology were performed in two mice and [(18)F]FDG uptake in the tumour and tumour size were correlated with the different cell fractions measured with flow cytometry in five mice. At d1/d3, similar reductions in [(18)F]FDG uptake and viable tumoral cell fraction were observed and these reductions preceded changes in tumour size. By d8/d10, [(18)F]FDG uptake had stabilised despite a further reduction in viable tumoral cell fraction. At these time points a major inflammatory response was observed. At d15, an increase in viable tumour cells was again observed and this was accurately predicted by an increase in [(18)F]FDG uptake, while the tumour volume remained unchanged. In contrast with variations in tumour volume, [(18)F]FDG is a good marker for chemotherapy response monitoring. However, optimal timing seems crucial since a transient increase in stromal reaction may result in overestimation of the fraction of viable cells.
URI: 
ISSN: 1619-7070
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Nuclear Medicine & Molecular Imaging
Clinical Genetics Section (-)
Clinical Residents Medicine
Hematology Section (-)
Radiopharmacy
Laboratory of Virology and Chemotherapy (Rega Institute)
Translational Cell & Tissue Research
Research Group Experimental Neurology
Laboratory for Cognitive Neurology
Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

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