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Title: NF-kappaB is involved in the regulation of CD154 (CD40 ligand) expression in primary human T cells
Authors: Srahna, Mohammed ×
Remacle, J E
Annamalai, K
Pype, Stefan
Huylebroeck, Danny
Boogaerts, Marc
Vandenberghe, Peter #
Issue Date: Aug-2001
Series Title: Clinical and Experimental Immunology vol:125 issue:2 pages:229-36
Abstract: Cognate interactions between CD154 (CD40 ligand, CD40L) on activated T cells and its receptor CD40 on various antigen-presenting cells are involved in thymus-dependent humoral immune responses and multiple other cell-mediated immune responses. We have studied the regulation of CD154 expression in human T cells after activation with anti-CD3 and anti-CD28 antibodies or after pharmacological activation of protein kinase C with phorbol 12-myristate 13-acetate, and the calcium ionophore ionomycin. Under these conditions, transcription of the CD154 gene was rapidly induced without requiring de novo protein synthesis. Pharmacological inhibitors of NF-kappaB activation down-regulated CD154 mRNA and protein levels. Cyclosporin A, an inhibitor of NF-AT activation, acted similarly, and the effects of both inhibitors were additive. A potential NF-kappaB binding site is present within the CD154 promoter at positions -1190 to - 1181. In electrophoretic mobility shift assays, this sequence was specifically bound by NF-kappaB present in nuclear extracts from activated T cells. Furthermore, in transient co-transfection of Jurkat T cells, p65 activated the transcription of a reporter construct containing a multimer of this NF-kappaB binding site. These observations demonstrate a role of NF-kappaB transcription factors in the regulation of CD40L expression in activated primary human T cells.
URI: 
ISSN: 0009-9104
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Biology (Celgen) (-)
Clinical Genetics Section (-)
Hematology Section (-)
Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

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