Author:

Keywords:

Arylamine N-Acetyltransferase, Base Sequence, Chromosome Mapping, Chromosomes, Human, Pair 8, Cloning, Molecular, Cosmids, DNA Primers, Humans, In Situ Hybridization, Fluorescence, Molecular Sequence Data, Polymorphism, Restriction Fragment Length, 0304 Medicinal and Biomolecular Chemistry, 0601 Biochemistry and Cell Biology, 1101 Medical Biochemistry and Metabolomics, Biochemistry & Molecular Biology, 3101 Biochemistry and cell biology, 3404 Medicinal and biomolecular chemistry

Abstract:

The NAT2 gene encodes for a polymorphic arylalkylamine N-acetyltransferase and thus accounts for the human N-acetylation polymorphism. By a NAT2-specific primer set we have screened a human chromosome 8-specific cosmid library. A positive cosmid clone was mapped by fluorescence in situ hybridization to 8p22. The polymerase chain reaction followed by restriction analysis of the PCR product was used to identify allele 2 to be contained in the cosmid clone.