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Title: The role of intracellular calcium stores in motilin induced contractions of the longitudinal muscle of the rabbit duodenum
Authors: Matthijs, Gert ×
Peeters, T L
Vantrappen, Gaston #
Issue Date: Jul-1989
Series Title: Naunyn-Schmiedeberg's archives of pharmacology. vol:339 issue:3 pages:332-9
Abstract: The contraction of longitudinal muscle strips of the rabbit duodenum in response to motilin and acetylcholine was investigated in normal and high K+-solutions in the presence and absence of external calcium, in order to demonstrate the existence of pharmaco-mechanical coupling for motilin and to examine whether the peptide mobilizes calcium from an intracellular store. In depolarized smooth muscle (140 mM K+), motilin (3.2 x 10(-9)-1 x 10(-7) M) and acetylcholine (1 x 10(-5) M) were still capable of causing a considerable, transient, concentration-dependent contraction in the presence of Ca2+. The 'extra'-contraction to motilin was not blocked by tetrodotoxin (1 microgram/ml) nor by atropine (10(-7) M), but acetylcholine (10(-5) M) was blocked by atropine. Verapamil (10(-7) M) could selectively block the K+ contraction without affecting the extra agonist contraction. Nitroprusside was ineffective up to 10(-4) M in high K+-solutions, but in normal Hepes-buffer it caused a concentration-dependent rightward shift of the concentration-response curve of motilin and acetylcholine contractions. In a calcium-depleted medium, high K+-depolarized muscle strips were still responsive to motilin and acetylcholine, but higher concentrations (10(-6) M) were needed than in the presence of calcium and the contractions reached only 57 +/- 11% and 74 +/- 9% respectively of the maximal contraction in 1.2 mM Ca2+ containing solutions. The response to motilin (10(-6) M) was not only smaller than that to acetylcholine (10(-5) M), it also faded more rapidly with time.(ABSTRACT TRUNCATED AT 250 WORDS)
ISSN: 0028-1298
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Clinical Genetics Section (-)
Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

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