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Title: Local origin and activity-dependent generation of nestin-expressing protoplasmic astrocytes in CA1
Authors: Kronenberg, Golo ×
Wang, Li-Ping
Geraerts, Martine
Babu, Harish
Synowitz, Michael
Vicens, Paloma
Lutsch, Gudrun
Glass, Rainer
Yamaguchi, Masahiro
Baekelandt, Veerle
Debyser, Zeger
Kettenmann, Helmut
Kempermann, Gerd #
Issue Date: Jul-2007
Publisher: Springer
Series Title: Brain Structure and Function vol:212 issue:1 pages:19-35
Abstract: Since reports that precursor cells in the adult subventricular zone (SVZ) contribute to regenerative neuro- and gliogenesis in CA1, we wondered whether a similar route of migration might also exist under physiological conditions. Permanent labeling of SVZ precursor cells with a lentiviral vector for green fluorescent protein did not reveal any migration from the SVZ into CA1 in the intact murine brain. However, in a nestin-GFP reporter mouse we found proliferating cells within the corpus callosum/alveus region expressing nestin and glial fibrillary acidic protein similar to precursor cells in the neighboring neurogenic region of the adult dentate gyrus. Within 3 weeks of BrdU administration, BrdU-positive nestin-GFP-expressing protoplasmic astrocytes emerged in CA1. Similar to precursor cells isolated from the dentate gyrus and the SVZ, nestin-GFP-expressing cells from corpus callosum/alveus were self-renewing and multipotent in vitro, whereas cells isolated from CA1 were not. Nestin-GFP-expressing cells in CA1 differentiated into postmitotic astrocytes characterized by S100beta expression. No new neurons were found in CA1. The number of nestin-GFP-expressing astrocytes in CA1 was increased by environmental enrichment. We conclude that astrogenesis in CA1 is influenced by environmental conditions. However, SVZ precursor cells do not contribute to physiological cellular plasticity in CA1.
URI: 
ISSN: 1863-2653
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Virology and Gene Therapy
Interdepartemental Stem Cell Institute (-)
Research Group for Neurobiology and Gene Therapy
× corresponding author
# (joint) last author

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